Culture methods

Culture Media based on Solid or Liquid

1. Solid medium - agar 1.5 -2
2. Semi-solid - agar <0.5 for motility
3. liquid medium = broth

Methods of Applying Organisms to Medium

1. Streak Culture(streak a bacteria) - Isolation from mixed population
2. lawn/Carpet culture(like garden full of bacteria,then add antibiotic to find suspectibilty)- Anti-biotic sensitivity

3. stab culture/puncture - O2 requirement,motility(crag's tube method)

4. Anaerobic Culture Method

   1. Candle Jar(old)
   2. Macintosh Field anaerobic jar
   3. Gas pak system(simple method chemical observes Oxygen from Air )

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Based on Composition of Nutrients

1. Basal medium(white rice) = BAsic nutrients no special nutrients

2. Complex Medium (multiple nutrients) eg:(brain heart infusion agar )

3. Defined Medium(exact amount of nutrients) = eg; ALkalline peptone water

BAsed on Nutrients for Particular Bacteria

1. Special Medium
   • Enriched Medium(enriched with spl items) , if known composition Defined medium , if unknown Complex medium
   • Enrichment Medium only in broth form (Only als growth of particular organisms)
   • Selective Medium only Solid form(help grown one Organism than other)
   • Differential medium(help differentiate organism) eg: urease medium , mc-conkey agar
   • Indicator medium (Indicates reaction) eg;Mc-Conkey color change
   • Transport Medium/Holding Medium (stop multiplying, stop dying) eg: cary-blair medium and VR medium for Cholera ,Pikes medium -strep.pyogenes,Stuart and Ame's for Gonococcal
   • Fermentation medium(Distinguish fermentation type)
   • For Anaerobes
     1. Robertson Cooked Meat medium
     2. PRAS - PRe reduced Anaerobicaly Sterilised Medium